A SYBR Green-based real-time RT-PCR assay to differentiate the H1N1 influenza virus lineages

Y Cong, Y Sun, X Deng, H Yu, X Lian… - Journal of Virological …, 2022 - Elsevier
… To evaluate the sensitivity of the qPCR method established in this study, a series of … real-time
RT-PCR assays to differentiate pandemic H1N1 2009 influenza viruses from swine H1N1 (…

Evaluation of a commercial colloidal gold assay for detection of influenza A and B virus in children's respiratory specimens

W Li, L Liu, L Chen, S Shang - Fetal and Pediatric Pathology, 2020 - Taylor & Francis
… The detection of influenza A and B viruses were performed in SLAN 96 P system with
commercial one-step real-time RT–PCR assay kit (Liferiver BioTech, Shanghai, China) cleared by …

A newly developed realtime PCR assay for discriminating influenza B virus Yamagata and Victoria lineages

J Wei, H Huang, H Qin, S Li, L Xing, Y Li… - Journal of Medical …, 2020 - Wiley Online Library
… RNA samples were tested using a commercial qRT-PCR kit specific … determination of
lineages and virus isolation, and samples positive for IAV were further subjected for the evaluation

[PDF][PDF] A multiplex TaqMan real-time RT-PCR assay for the simultaneous detection of H4, H6, and H10 avian influenza viruses

F Yang, S Yan, L Zhu, H Yao, D Dong, D Wu, N Wu… - Heliyon, 2023 - cell.com
… 2021 and evaluated previously using traditional 93 virus isolation in … 94 Thirty-two nose
and/or throat swabs from humans … and evaluated using a commercial Influenza A and B Virus 96 …

Multichannel immunosensor platform for the rapid detection of SARS-CoV-2 and influenza A (H1N1) virus

J Li, R Lin, Y Yang, R Zhao, S Song… - … applied materials & …, 2021 - ACS Publications
… (31) The surface plasmon resonance is label-free and real-time but normally … commercial
ELISA. The established biosensing platform was evaluated using the HA proteins of A(H1N1

Evaluation of enterovirus nucleic acids detection method based on ultra‐fast realtime fluorescence RT‐PCR technology—A pilot study

X Gao, J Hao, L Yu, Y Cao, J Liang… - Journal of Medical …, 2022 - Wiley Online Library
… -polymerase chain reaction (RT-PCR) equipment, this study aimed to evaluate the … nucleic
acids based on ultra-fast real-time fluorescence RT-PCR technology. A total of 61 cases were …

Application of a ddRT-PCR to quantify seasonal influenza virus for viral isolation

Y Liu, J Zhao, X Peng, G Lu, W Shi, Z Feng… - Biosafety and …, 2022 - mednexus.org
… in the influenza monitoring network in Beijing. Each laboratory is responsible every week …
real-time polymerase chain reaction (ddRT-PCR) approach was introduced to quantify the viral

A novel assay based on DNA melting temperature for multiplexed identification of SARS-CoV-2 and influenza A/B viruses

P Gao, Y Fan, X Kong, R Zhang, L Chen… - Frontiers in …, 2023 - frontiersin.org
… , stability, RNase resistance, non-infectivity and inability to replicate by itself both in vivo and
… were used to evaluate the LOD and precision of 7 commercial real-time PCR kits for Zaire …

[HTML][HTML] A high-throughput, multi-index isothermal amplification platform for rapid detection of 19 types of common respiratory viruses including SARS-CoV-2

W Xing, Y Liu, H Wang, S Li, Y Lin, L Chen, Y Zhao… - Engineering, 2020 - Elsevier
detected and displayed on the computer screen in real time. … targets for the evaluation of
the system repeatability. First, … of 18 types of respiratory viruses in Beijing by analyzing a large …

Reliability of real-time RT-PCR tests to detect SARS-Cov-2: A literature review

C Bezier, G Anthoine, A Charki - International Journal of …, 2020 - metrology-journal.org
… -2 and the commercial primer, including with genes selected in real-time PCR detection tests
… inactivation methods based on 23 confirmed cases of COVID-19 in Beijing, 23 samples of …